Isolation and preliminary characterization of temperature-sensitive mutants of poliovirus type 1.

We isolated six temperature-sensitive mutants of poliovirus type 1 (Mahoney) by hydroxylamine mutagenesis and replica plating at 31, 33 (permissive), and 39 degrees C (restrictive). One of these mutants, designated tsB9, was chosen for more detailed examination. tsB9 accumulated 25% of the wild-type...

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Detalhes bibliográficos
Publicado no:J Virol
Main Authors: Hewlett, M J, Axelrod, J H, Antinoro, N, Feld, R
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 1982
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC256848/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6284963/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jvi.41.3.1089-1094.1982
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