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Tuning Escherichia coli for membrane protein overexpression
A simple generic method for optimizing membrane protein overexpression in Escherichia coli is still lacking. We have studied the physiological response of the widely used “Walker strains” C41(DE3) and C43(DE3), which are derived from BL21(DE3), to membrane protein overexpression. For unknown reasons...
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主要な著者: | , , , , , , , , , , |
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フォーマット: | Artigo |
言語: | Inglês |
出版事項: |
National Academy of Sciences
2008
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主題: | |
オンライン・アクセス: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2567230/ https://ncbi.nlm.nih.gov/pubmed/18796603 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0804090105 |
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