A scalable, GFP-based pipeline for membrane protein overexpression screening and purification

We describe a generic, GFP-based pipeline for membrane protein overexpression and purification in Escherichia coli. We exemplify the use of the pipeline by the identification and characterization of E. coli YedZ, a new, membrane-integral flavocytochrome. The approach is scalable and suitable for hig...

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Detalhes bibliográficos
Main Authors: Drew, David, Slotboom, Dirk-Jan, Friso, Giulia, Reda, Torsten, Genevaux, Pierre, Rapp, Mikaela, Meindl-Beinker, Nadja M., Lambert, Wietske, Lerch, Mirjam, Daley, Daniel O., Van Wijk, Klaas-Jan, Hirst, Judy, Kunji, Edmund, De Gier, Jan-Willem
Formato: Artigo
Idioma:Inglês
Publicado em: Cold Spring Harbor Laboratory Press 2005
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2279312/
https://ncbi.nlm.nih.gov/pubmed/15987891
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1110/ps.051466205
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