Tuning Escherichia coli for membrane protein overexpression

A simple generic method for optimizing membrane protein overexpression in Escherichia coli is still lacking. We have studied the physiological response of the widely used “Walker strains” C41(DE3) and C43(DE3), which are derived from BL21(DE3), to membrane protein overexpression. For unknown reasons...

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Bibliographic Details
Main Authors:	Wagner, Samuel, Klepsch, Mirjam M., Schlegel, Susan, Appel, Ansgar, Draheim, Roger, Tarry, Michael, Högbom, Martin, van Wijk, Klaas J., Slotboom, Dirk J., Persson, Jan O., de Gier, Jan-Willem
Format:	Artigo
Language:	Inglês
Published:	National Academy of Sciences 2008
Subjects:	Biological Sciences
Online Access:	https://ncbi.nlm.nih.gov/pmc/articles/PMC2567230/ https://ncbi.nlm.nih.gov/pubmed/18796603 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0804090105
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Tuning Escherichia coli for membrane protein overexpression

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