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The Affinity Grid: A pre-fabricated EM grid for monolayer purification
We have recently developed “monolayer purification” as a rapid and convenient technique to produce specimens of His-tagged proteins or macromolecular complexes for single particle electron microscopy (EM) without prior biochemical purification. Here, we introduce the “Affinity Grid”, a pre-fabricate...
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| Main Authors: | , , , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
2008
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2564605/ https://ncbi.nlm.nih.gov/pubmed/18655791 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jmb.2008.07.023 |
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