טוען...
Monolayer purification: A rapid method for isolating protein complexes for single-particle electron microscopy
Visualizing macromolecular complexes by single-particle electron microscopy (EM) entails stringent biochemical purification, specimen preparation, low-dose imaging, and 3D image reconstruction. Here, we introduce the “monolayer purification” method, which employs nickel-nitrilotriacetic acid (Ni-NTA...
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| Main Authors: | , , |
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| פורמט: | Artigo |
| שפה: | Inglês |
| יצא לאור: |
National Academy of Sciences
2008
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| נושאים: | |
| גישה מקוונת: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2290746/ https://ncbi.nlm.nih.gov/pubmed/18347330 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0800867105 |
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