In vivo incorporation of unnatural amino acids to probe structure, dynamics and ligand binding in a large protein by Nuclear Magnetic Resonance spectroscopy

In vivo incorporation of isotopically labeled unnatural amino acids into large proteins drastically reduces the complexity of nuclear magnetic resonance (NMR) spectra. Incorporation is accomplished by co-expressing an orthogonal tRNA/aminoacyl-tRNA synthetase pair specific for the unnatural amino ac...

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Auteurs principaux: Cellitti, Susan E., Jones, David H., Lagpacan, Leanna, Hao, Xueshi, Zhang, Qiong, Hu, Huiyong, Brittain, Scott M., Brinker, Achim, Caldwell, Jeremy, Bursulaya, Badry, Spraggon, Glen, Brock, Ansgar, Ryu, Youngha, Uno, Tetsuo, Schultz, Peter G., Geierstanger, Bernhard H.
Format: Artigo
Langue:Inglês
Publié: 2008
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Article
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC2562865/
https://ncbi.nlm.nih.gov/pubmed/18576636
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ja801602q
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