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γ-Glutamyl Hydrolase: Kinetic Characterization of Isopeptide Hydrolysis Using Fluorogenic Substrates

γ-Glutamyl hydrolase, a cysteine peptidase, catalyzes the hydrolysis of poly-γ-glutamate derivatives of folate co-factors and many antifolate drugs. We have used internally quenched fluorogenic derivatives of glutamyl-γ-glutamate and (4,4-difluoro)glutamyl-γ-glutamate to examine the effect of fluori...

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Hlavní autoři: Alexander, Jessica P., Ryan, Thomas J., Ballou, David P., Coward, James K.
Médium: Artigo
Jazyk:Inglês
Vydáno: 2008
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC2562270/
https://ncbi.nlm.nih.gov/pubmed/18171026
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi701607v
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