Use of shotgun proteomics for the identification, confirmation, and correction of C. elegans gene annotations

We describe a general mass spectrometry-based approach for gene annotation of any organism and demonstrate its effectiveness using the nematode Caenorhabditis elegans. We detected 6779 C. elegans proteins (67,047 peptides), including 384 that, although annotated in WormBase WS150, lacked cDNA or oth...

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Detalhes bibliográficos
Main Authors: Merrihew, Gennifer E., Davis, Colleen, Ewing, Brent, Williams, Gary, Käll, Lukas, Frewen, Barbara E., Noble, William Stafford, Green, Phil, Thomas, James H., MacCoss, Michael J.
Formato: Artigo
Idioma:Inglês
Publicado em: Cold Spring Harbor Laboratory Press 2008
Assuntos:
Methods
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2556273/
https://ncbi.nlm.nih.gov/pubmed/18653799
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1101/gr.077644.108
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