Use of shotgun proteomics for the identification, confirmation, and correction of C. elegans gene annotations

We describe a general mass spectrometry-based approach for gene annotation of any organism and demonstrate its effectiveness using the nematode Caenorhabditis elegans. We detected 6779 C. elegans proteins (67,047 peptides), including 384 that, although annotated in WormBase WS150, lacked cDNA or oth...

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Autores principales: Merrihew, Gennifer E., Davis, Colleen, Ewing, Brent, Williams, Gary, Käll, Lukas, Frewen, Barbara E., Noble, William Stafford, Green, Phil, Thomas, James H., MacCoss, Michael J.
Formato: Artigo
Lenguaje:Inglês
Publicado: Cold Spring Harbor Laboratory Press 2008
Materias:
Methods
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC2556273/
https://ncbi.nlm.nih.gov/pubmed/18653799
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1101/gr.077644.108
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