Prevention of Amino Acid Conversion in SILAC Experiments with Embryonic Stem Cells

Recent studies using stable isotope labeling with amino acids in culture (SILAC) in quantitative proteomics have made mention of the problematic conversion of isotope-coded arginine to proline in cells. The resulting converted proline peptide divides the heavy peptide ion signal causing inaccuracy w...

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Hlavní autoři: Bendall, Sean C., Hughes, Chris, Stewart, Morag H., Doble, Brad, Bhatia, Mickie, Lajoie, Gilles A.
Médium: Artigo
Jazyk:Inglês
Vydáno: American Society for Biochemistry and Molecular Biology 2008
Témata:
Research
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC2556023/
https://ncbi.nlm.nih.gov/pubmed/18487603
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/mcp.M800113-MCP200
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