Assessing the impact of minimizing arginine conversion in fully defined SILAC culture medium in human embryonic stem cells

We present a fully defined culture system (adapted Essential8(TM) [E8(TM)] medium in combination with vitronectin) for human embryonic stem cells that can be used for SILAC purposes. Although a complete incorporation of the labels was observed after 4 days in culture, over 90% of precursors showed a...

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Detalhes bibliográficos
Publicado no:Proteomics
Main Authors: Scheerlinck, Ellen, Van Steendam, Katleen, Daled, Simon, Govaert, Elisabeth, Vossaert, Liesbeth, Meert, Paulien, Van Nieuwerburgh, Filip, Van Soom, Ann, Peelman, Luc, De Sutter, Petra, Heindryckx, Björn, Dhaenens, Maarten, Deforce, Dieter
Formato: Artigo
Idioma:Inglês
Publicado em: John Wiley and Sons Inc. 2016
Assuntos:
Technology
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5096064/
https://ncbi.nlm.nih.gov/pubmed/27392809
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/pmic.201600174
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