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Efficient production of membrane-integrated and detergent-soluble G protein-coupled receptors in Escherichia coli
G protein-coupled receptors (GPCRs) are notoriously difficult to express, particularly in microbial systems. Using GPCR fusions with the green fluorescent protein (GFP), we conducted studies to identify bacterial host effector genes that result in a general and significant enhancement in the amount...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Cold Spring Harbor Laboratory Press
2008
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2548370/ https://ncbi.nlm.nih.gov/pubmed/18593817 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1110/ps.035980.108 |
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