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Efficient production of membrane-integrated and detergent-soluble G protein-coupled receptors in Escherichia coli

G protein-coupled receptors (GPCRs) are notoriously difficult to express, particularly in microbial systems. Using GPCR fusions with the green fluorescent protein (GFP), we conducted studies to identify bacterial host effector genes that result in a general and significant enhancement in the amount...

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Bibliografiske detaljer
Main Authors: Link, A. James, Skretas, Georgios, Strauch, Eva-Maria, Chari, Nandini S., Georgiou, George
Format: Artigo
Sprog:Inglês
Udgivet: Cold Spring Harbor Laboratory Press 2008
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2548370/
https://ncbi.nlm.nih.gov/pubmed/18593817
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1110/ps.035980.108
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