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Fluorescent Single-Stranded DNA Binding Protein as a Probe for Sensitive, Real-Time Assays of Helicase Activity

The formation and maintenance of single-stranded DNA (ssDNA) are essential parts of many processes involving DNA. For example, strand separation of double-stranded DNA (dsDNA) is catalyzed by helicases, and this exposure of the bases on the DNA allows further processing, such as replication, recombi...

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Bibliografiske detaljer
Main Authors: Dillingham, Mark S., Tibbles, Katherine L., Hunter, Jackie L., Bell, Jason C., Kowalczykowski, Stephen C., Webb, Martin R.
Format: Artigo
Sprog:Inglês
Udgivet: The Biophysical Society 2008
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2547451/
https://ncbi.nlm.nih.gov/pubmed/18599625
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1529/biophysj.108.133512
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