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Detection of Yersinia pestis in Sputum by Real-Time PCR

A 5′ nuclease PCR assay for detection of the Yersinia pestis plasminogen activator (pla) gene in human respiratory specimens with simulated Y. pestis infection was developed. An internal positive control was added to the reaction mixture in order to detect the presence of PCR inhibitors that are oft...

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Detalles Bibliográficos
Main Authors: Loïez, Caroline, Herwegh, Stéphanie, Wallet, Frédéric, Armand, Sylvie, Guinet, Françoise, Courcol, René J.
Formato: Artigo
Idioma:Inglês
Publicado: American Society for Microbiology 2003
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC254301/
https://ncbi.nlm.nih.gov/pubmed/14532247
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.41.10.4873-4875.2003
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