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Detection of Yersinia pestis in Sputum by Real-Time PCR

A 5′ nuclease PCR assay for detection of the Yersinia pestis plasminogen activator (pla) gene in human respiratory specimens with simulated Y. pestis infection was developed. An internal positive control was added to the reaction mixture in order to detect the presence of PCR inhibitors that are oft...

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Detalhes bibliográficos
Main Authors: Loïez, Caroline, Herwegh, Stéphanie, Wallet, Frédéric, Armand, Sylvie, Guinet, Françoise, Courcol, René J.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2003
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC254301/
https://ncbi.nlm.nih.gov/pubmed/14532247
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.41.10.4873-4875.2003
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