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Development and Use of an Internal Positive Control for Detection of Bordetella pertussis by PCR

An internal control of amplification was constructed by recombinant PCR to detect PCR inhibitors. This exogenous DNA was included in the reaction mixture and coamplified with the target gene. This detection was successfully applied to the diagnosis of whooping cough by amplification of a fragment of...

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Detalhes bibliográficos
Main Authors: Herwegh, Stéphanie, Carnoy, Christophe, Wallet, Frédéric, Loïez, Caroline, Courcol, René J.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2005
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1153740/
https://ncbi.nlm.nih.gov/pubmed/15872283
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.43.5.2462-2464.2005
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