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Development and Use of an Internal Positive Control for Detection of Bordetella pertussis by PCR
An internal control of amplification was constructed by recombinant PCR to detect PCR inhibitors. This exogenous DNA was included in the reaction mixture and coamplified with the target gene. This detection was successfully applied to the diagnosis of whooping cough by amplification of a fragment of...
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| Autori principali: | , , , , |
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| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
American Society for Microbiology
2005
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1153740/ https://ncbi.nlm.nih.gov/pubmed/15872283 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.43.5.2462-2464.2005 |
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