Poliovirus proteinase 3C: large-scale expression, purification, and specific cleavage activity on natural and synthetic substrates in vitro.

Proteinase 3C of poliovirus type 2 (Sabin) was expressed at 4% total protein in Escherichia coli. The protein was soluble and could be purified by a simple scheme. It was weakly active on the capsid precursor P1 (expressed in vitro), which contains two cleavage sites. The products of processing P1 w...

ver descrição completa

Detalhes bibliográficos

Publicado no:	J Virol
Principais autores:	Nicklin, M J, Harris, K S, Pallai, P V, Wimmer, E
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 1988
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC254243/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2846872/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jvi.62.12.4586-4593.1988
Tags:	Adicionar Tag Sem tags, seja o primeiro a adicionar uma tag!