Poliovirus proteinase 3C: large-scale expression, purification, and specific cleavage activity on natural and synthetic substrates in vitro.

Proteinase 3C of poliovirus type 2 (Sabin) was expressed at 4% total protein in Escherichia coli. The protein was soluble and could be purified by a simple scheme. It was weakly active on the capsid precursor P1 (expressed in vitro), which contains two cleavage sites. The products of processing P1 w...

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Vydáno v:	J Virol
Hlavní autoři:	Nicklin, M J, Harris, K S, Pallai, P V, Wimmer, E
Médium:	Artigo
Jazyk:	Inglês
Vydáno:	American Society for Microbiology (ASM) 1988
Témata:	Research Article
On-line přístup:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC254243/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2846872/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jvi.62.12.4586-4593.1988
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