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The structure of SgrAI bound to DNA; recognition of an 8 base pair target
The three-dimensional X-ray crystal structure of the ‘rare cutting’ type II restriction endonuclease SgrAI bound to cognate DNA is presented. SgrAI forms a dimer bound to one duplex of DNA. Two Ca(2+) bind in the enzyme active site, with one ion at the interface between the protein and DNA, and the...
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| Autors principals: | , , , , , , , |
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| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
Oxford University Press
2008
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2532715/ https://ncbi.nlm.nih.gov/pubmed/18701646 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkn510 |
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