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Rational vector design and multi-pathway modulation of HEK 293E cells yield recombinant antibody titers exceeding 1 g/l by transient transfection under serum-free conditions

Transient transfection allows for fast production of recombinant proteins. However, the current bottlenecks in transient transfection are low titers and low specific productivity compared to stable cell lines. Here, we report an improved transient transfection protocol that yields titers exceeding 1...

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Hlavní autoři: Backliwal, Gaurav, Hildinger, Markus, Chenuet, Sebastien, Wulhfard, Sarah, De Jesus, Maria, Wurm, Florian M.
Médium: Artigo
Jazyk:Inglês
Vydáno: Oxford University Press 2008
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC2528171/
https://ncbi.nlm.nih.gov/pubmed/18617574
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkn423
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