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Proteolytic Fragments of Insulysin (IDE) Retain Substrate Binding, But Lose Allosteric Regulation
Treatment of an N-terminal containing His(6) tagged insulysin (His(6)-IDE) with proteinase K led to the initial cleavage of the His tag and linker region. This was followed by C-terminal cleavages resulting in intermediate fragments of ~95 kDa and ~76 kDa and finally a relatively stable ~56 kDa frag...
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| Hauptverfasser: | , , , |
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| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
2006
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2519894/ https://ncbi.nlm.nih.gov/pubmed/17154546 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi061298u |
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