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Proteolytic Fragments of Insulysin (IDE) Retain Substrate Binding, But Lose Allosteric Regulation

Treatment of an N-terminal containing His(6) tagged insulysin (His(6)-IDE) with proteinase K led to the initial cleavage of the His tag and linker region. This was followed by C-terminal cleavages resulting in intermediate fragments of ~95 kDa and ~76 kDa and finally a relatively stable ~56 kDa frag...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Song, Eun Suk, Cady, Clint, Fried, Michael G., Hersh, Louis B.
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2006
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2519894/
https://ncbi.nlm.nih.gov/pubmed/17154546
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi061298u
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