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LOCATING THE RATE-DETERMINING STEP(S) FOR THREE-STEP HYDROLASE-CATALYZED REACTIONS WITH DYNAFIT
Hydrolytic reactions of oligopeptide 4-nitroanilides catalyzed by human-α-thrombin, human activated protein C and human factor Xa were studied at pH 8.0–8.4 and 25.0 ± 0.1 °C by the progress curve method and individual rate constants were calculated mostly within 10% internal error using DYNAFITV. A...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2008
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2519142/ https://ncbi.nlm.nih.gov/pubmed/18342021 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bbapap.2008.02.004 |
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