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Passaging HuES Human Embryonic Stem Cell-lines with Trypsin

In this video we demonstrate how our lab routinely passages HuES human embryonic stem cell lines with trypsin. Human embryonic stem cells are artifacts of cell culture, and tend to acquire karyotypic abnormalities with high population doublings. Proper passaging is essential for maintaining a health...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Hauptverfasser: Trish, Erin, Dimos, John, Eggan, Kevin
Format: Artigo
Sprache:Inglês
Veröffentlicht: MyJove Corporation 2006
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2504455/
https://ncbi.nlm.nih.gov/pubmed/18704181
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/49
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