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Passaging HuES Human Embryonic Stem Cell-lines with Trypsin

In this video we demonstrate how our lab routinely passages HuES human embryonic stem cell lines with trypsin. Human embryonic stem cells are artifacts of cell culture, and tend to acquire karyotypic abnormalities with high population doublings. Proper passaging is essential for maintaining a health...

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Detalhes bibliográficos
Main Authors: Trish, Erin, Dimos, John, Eggan, Kevin
Formato: Artigo
Idioma:Inglês
Publicado em: MyJove Corporation 2006
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2504455/
https://ncbi.nlm.nih.gov/pubmed/18704181
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/49
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