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Human ES cells: Starting Culture from Frozen Cells

Here we demonstrate how our lab begins a HuES human embryonic stem cell line culture from a frozen stock. First, a one to two day old ten cm plate of approximately one (to two) million irradiated mouse embryonic fibroblast feeder cells is rinsed with HuES media to remove residual serum and cell debr...

詳細記述

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書誌詳細
主要な著者: Trish, Erin, Dimos, John, Eggan, Kevin
フォーマット: Artigo
言語:Inglês
出版事項: MyJove Corporation 2006
主題:
オンライン・アクセス:https://ncbi.nlm.nih.gov/pmc/articles/PMC2504448/
https://ncbi.nlm.nih.gov/pubmed/18704174
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/86
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