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Direct Measurement of Acetylesterase in Living Protist Cells

The fluorogenic acetylesterase (acetic ester hydrolase EC 3.1.1.6.) substrate, fluorescein diacetate, was used to measure enzyme activity in living protist cells. The visual enzyme assay was done by monitoring fluorochromasia by fluorescent microscopy. Quantitative fluorogenic assays were done by me...

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Bibliografske podrobnosti
izdano v:J Bacteriol
Principais autores: Medzon, Edward L., Brady, Marilyn L.
Format: Artigo
Jezik:Inglês
Izdano: American Society for Microbiology (ASM) 1969
Teme:
Online dostop:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC249621/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/4974398/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.97.1.402-415.1969
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