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Direct Measurement of Acetylesterase in Living Protist Cells

The fluorogenic acetylesterase (acetic ester hydrolase EC 3.1.1.6.) substrate, fluorescein diacetate, was used to measure enzyme activity in living protist cells. The visual enzyme assay was done by monitoring fluorochromasia by fluorescent microscopy. Quantitative fluorogenic assays were done by me...

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Bibliographic Details
Published in:J Bacteriol
Main Authors: Medzon, Edward L., Brady, Marilyn L.
Format: Artigo
Language:Inglês
Published: American Society for Microbiology (ASM) 1969
Subjects:
Online Access:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC249621/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/4974398/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.97.1.402-415.1969
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