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Direct Measurement of Acetylesterase in Living Protist Cells

The fluorogenic acetylesterase (acetic ester hydrolase EC 3.1.1.6.) substrate, fluorescein diacetate, was used to measure enzyme activity in living protist cells. The visual enzyme assay was done by monitoring fluorochromasia by fluorescent microscopy. Quantitative fluorogenic assays were done by me...

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Podrobná bibliografie
Vydáno v:J Bacteriol
Hlavní autoři: Medzon, Edward L., Brady, Marilyn L.
Médium: Artigo
Jazyk:Inglês
Vydáno: American Society for Microbiology (ASM) 1969
Témata:
On-line přístup:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC249621/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/4974398/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.97.1.402-415.1969
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