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Hydrogen location in stages of an enzyme-catalyzed reaction: time-of-flight neutron structure of d-xylose isomerase with bound d-xylulose†

The time-of-flight neutron Laue technique has been used to determine the location of hydrogen atoms in the enzyme D-xylose isomerase (XI). The neutron structure of crystalline XI with bound product, D-xylulose, shows, unexpectedly, that O5 of D-xylulose is not protonated but is hydrogen-bonded to do...

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Detalhes bibliográficos
Main Authors: Kovalevsky, Andrey Y., Katz, Amy K., Carrell, H. L., Hanson, Leif, Mustyakimov, Marat, Fisher, S. Zoe, Coates, Leighton, Schoenborn, Benno P., Bunick, Gerard J., Glusker, Jenny. P., Langan, Paul
Formato: Artigo
Idioma:Inglês
Publicado em: 2008
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2488401/
https://ncbi.nlm.nih.gov/pubmed/18578508
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi8005434
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