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New Centrifugation Technique for Isolating Enzymes from Large Cell Structures: Isolation and Characterization of Two Bacillus subtilis Autolysins
Bacillus subtilis cell walls can be centrifuged through a linear gradient of 0 to 2 m LiCl and 10 to 25% sucrose so that different autolysins are removed by different salt concentrations and banded in separate positions as the walls pass through the gradient. Using this technique we have found that...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
1972
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC247350/ https://ncbi.nlm.nih.gov/pubmed/4622131 |
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