New Centrifugation Technique for Isolating Enzymes from Large Cell Structures: Isolation and Characterization of Two Bacillus subtilis Autolysins
Bacillus subtilis cell walls can be centrifuged through a linear gradient of 0 to 2 m LiCl and 10 to 25% sucrose so that different autolysins are removed by different salt concentrations and banded in separate positions as the walls pass through the gradient. Using this technique we have found that...
Guardat en:
| Publicat a: | J Bacteriol |
|---|---|
| Autors principals: | , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
American Society for Microbiology (ASM)
1972
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC247350/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/4622131/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.109.3.1258-1265.1972 |
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