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New Centrifugation Technique for Isolating Enzymes from Large Cell Structures: Isolation and Characterization of Two Bacillus subtilis Autolysins
Bacillus subtilis cell walls can be centrifuged through a linear gradient of 0 to 2 m LiCl and 10 to 25% sucrose so that different autolysins are removed by different salt concentrations and banded in separate positions as the walls pass through the gradient. Using this technique we have found that...
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| Publicado no: | J Bacteriol |
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| Main Authors: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
American Society for Microbiology (ASM)
1972
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC247350/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/4622131/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.109.3.1258-1265.1972 |
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