Rapid Method for Isolation of Large Quantities of Outer Membrane from Escherichia coli K-12 and Its Application to the Study of Envelope Mutants

A rapid method for the isolation of large quantities of bacterial outer membrane is described. This cell envelope component was removed from plasmolyzed cells of Escherichia coli K-12 by lysozyme-ethylenediaminetetra-acetic acid treatment, aggregated by lowering the pH to 5.0, and recovered by centr...

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Pubblicato in:	J Bacteriol
Autori principali:	Wolf-Watz, Hans, Normark, Staffan, Bloom, Gunnar D.
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	American Society for Microbiology (ASM) 1973
Soggetti:	Morphology and Ultrastructure
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC246369/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/4199509/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.115.3.1191-1197.1973
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