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Rapid Method for Isolation of Large Quantities of Outer Membrane from Escherichia coli K-12 and Its Application to the Study of Envelope Mutants

A rapid method for the isolation of large quantities of bacterial outer membrane is described. This cell envelope component was removed from plasmolyzed cells of Escherichia coli K-12 by lysozyme-ethylenediaminetetra-acetic acid treatment, aggregated by lowering the pH to 5.0, and recovered by centr...

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Detalhes bibliográficos
Main Authors: Wolf-Watz, Hans, Normark, Staffan, Bloom, Gunnar D.
Formato: Artigo
Idioma:Inglês
Publicado em: 1973
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC246369/
https://ncbi.nlm.nih.gov/pubmed/4199509
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