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Rapid Method for Isolation of Large Quantities of Outer Membrane from Escherichia coli K-12 and Its Application to the Study of Envelope Mutants

A rapid method for the isolation of large quantities of bacterial outer membrane is described. This cell envelope component was removed from plasmolyzed cells of Escherichia coli K-12 by lysozyme-ethylenediaminetetra-acetic acid treatment, aggregated by lowering the pH to 5.0, and recovered by centr...

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Bibliographische Detailangaben
Veröffentlicht in:J Bacteriol
Hauptverfasser: Wolf-Watz, Hans, Normark, Staffan, Bloom, Gunnar D.
Format: Artigo
Sprache:Inglês
Veröffentlicht: American Society for Microbiology (ASM) 1973
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC246369/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/4199509/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.115.3.1191-1197.1973
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