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Structural requirements of tetracycline-Tet repressor interaction: determination of equilibrium binding constants for tetracycline analogs with the Tet repressor.

We used the Tn10-encoded Tet repressor, which has a highly specific binding capacity for tetracycline, to probe contacts between the drug and protein by chemical interference studies of the antibiotic. For that purpose, the equilibrium association constants of modified tetracyclines with the Tet rep...

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Autors principals: Degenkolb, J, Takahashi, M, Ellestad, G A, Hillen, W
Format: Artigo
Idioma:Inglês
Publicat: 1991
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC245224/
https://ncbi.nlm.nih.gov/pubmed/1929330
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