Semirational design of active tumor suppressor p53 DNA binding domain with enhanced stability

We have designed a p53 DNA binding domain that has virtually the same binding affinity for the gadd45 promoter as does wild-type protein but is considerably more stable. The design strategy was based on molecular evolution of the protein domain. Naturally occurring amino acid substitutions were iden...

ver descrição completa

Detalhes bibliográficos

Publicado no:	Proc Natl Acad Sci U S A
Principais autores:	Nikolova, Penka V., Henckel, Julia, Lane, David P., Fersht, Alan R.
Formato:	Artigo
Idioma:	Inglês
Publicado em:	National Academy of Sciences 1998
Assuntos:	Biological Sciences
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC24508/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9843948/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.95.25.14675
Tags:	Adicionar Tag Sem tags, seja o primeiro a adicionar uma tag!