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Quadruplex Real-Time PCR Assay Using Allele-Specific Scorpion Primers for Detection of Mutations Conferring Clarithromycin Resistance to Helicobacter pylori

We developed a single-vessel multiplex real-time PCR assay that detects Helicobacter pylori infection and identified the four existing alleles of the 23S rRNA genes of H. pylori—the wild-type sequence and the three mutations conferring clarithromycin resistance—using allele-specific Scorpion primers...

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Detalhes bibliográficos
Main Authors: Burucoa, Christophe, Garnier, Martine, Silvain, Christine, Fauchère, Jean-Louis
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 2008
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2446943/
https://ncbi.nlm.nih.gov/pubmed/18463216
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.02352-07
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