PCR Using 3′-Mismatched Primers To Detect A2142C Mutation in 23S rRNA Conferring Resistance to Clarithromycin in Helicobacter pylori Clinical Isolates

Twenty-five clarithromycin-resistant Helicobacter pylori strains (selected by agar dilution) were studied to detect A2142G and A2143G mutations in the 23S rRNA gene by a PCR-restriction fragment length polymorphism method and an A2142C mutation by PCR using a 3′-mismatched specific primer. A 700-bp...

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Hlavní autoři: Alarcón, Teresa, Domingo, Diego, Prieto, Nuria, López-Brea, Manuel
Médium: Artigo
Jazyk:Inglês
Vydáno: American Society for Microbiology 2000
Témata:
Bacteriology
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC86249/
https://ncbi.nlm.nih.gov/pubmed/10655418
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