PCR Using 3′-Mismatched Primers To Detect A2142C Mutation in 23S rRNA Conferring Resistance to Clarithromycin in Helicobacter pylori Clinical Isolates

Twenty-five clarithromycin-resistant Helicobacter pylori strains (selected by agar dilution) were studied to detect A2142G and A2143G mutations in the 23S rRNA gene by a PCR-restriction fragment length polymorphism method and an A2142C mutation by PCR using a 3′-mismatched specific primer. A 700-bp...

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Detalles Bibliográficos
Main Authors: Alarcón, Teresa, Domingo, Diego, Prieto, Nuria, López-Brea, Manuel
Formato: Artigo
Idioma:Inglês
Publicado: American Society for Microbiology 2000
Assuntos:
Bacteriology
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC86249/
https://ncbi.nlm.nih.gov/pubmed/10655418
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