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Comparison of Real-Time PCR for Detection of the tcdC Gene with Four Toxin Immunoassays and Culture in Diagnosis of Clostridium difficile Infection

We have developed a rapid real-time PCR method using fluorescence resonance energy transfer probes and the LightCycler (Roche Diagnostics), which will detect the presence of the tcdC gene of Clostridium difficile in stool samples. Our PCR method also will identify the presence of base pair deletions...

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Detalhes bibliográficos
Main Authors: Sloan, Lynne M., Duresko, Brian J., Gustafson, Daniel R., Rosenblatt, Jon E.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 2008
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2446834/
https://ncbi.nlm.nih.gov/pubmed/18434563
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.00032-08
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