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A novel universal real-time PCR system using the attached universal duplex probes for quantitative analysis of nucleic acids

BACKGROUND: Real-time PCR techniques are being widely used for nucleic acids analysis, but one limitation of current frequently employed real-time PCR is the high cost of the labeled probe for each target molecule. RESULTS: We describe a real-time PCR technique employing attached universal duplex pr...

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Main Authors: Yang, Litao, Liang, Wanqi, Jiang, Lingxi, Li, Wenquan, Cao, Wei, Wilson, Zoe A, Zhang, Dabing
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2008
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2443376/
https://ncbi.nlm.nih.gov/pubmed/18522756
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1471-2199-9-54
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