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Enhancing the specificity of the enterokinase cleavage reaction to promote efficient cleavage of a fusion tag
In our work with designed minimalist proteins based on the bZIP motif, we have found our His-tagged proteins to be prone to inclusion body formation and aggregation; we suspect this problem is largely due to the His tag, known to promote aggregation. Using AhR6-C/EBP, a hybrid of the AhR basic regio...
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| Main Authors: | , , , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
2008
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2441903/ https://ncbi.nlm.nih.gov/pubmed/18406169 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.pep.2008.02.015 |
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