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Steady-State Kinetics and Mechanism of LpxD, the N-Acyltransferase of Lipid A Biosynthesis
LpxD catalyzes the third step of lipid A biosynthesis, the R-3-hydroxymyristoyl-acyl carrier protein (R-3-OHC(14)-ACP)-dependent N-acylation of UDP-3-O-(R-3-hydroxymyristoyl)-α-D-glucosamine [UDP-3-O-(R-3-OHC(14))-GlcN]. We have now over-expressed and purified E. coli LpxD to homogeneity. Steady sta...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
2008
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2435086/ https://ncbi.nlm.nih.gov/pubmed/18422345 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi800240r |
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