Improved lysis of group N streptococci for isolation and rapid characterization of plasmid deoxyribonucleic acid.
Procedures for effective cellular lysis and plasmid deoxyribonucleic acid (DNA) isolation from group N streptococci were developed. Cells were grown at 32 degrees C for 4 h in a modified Elliker broth containing 20 mM DL-threonine. After cellular digestion with 2 mg of lysozyme per ml for 7 min at 3...
Salvato in:
| Pubblicato in: | Appl Environ Microbiol |
|---|---|
| Autori principali: | , , |
| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
American Society for Microbiology (ASM)
1978
|
| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC242884/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/416755/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.35.3.592-600.1978 |
| Tags: |
Nessun Tag, puoi essere il primo ad aggiungerne!!
|