Improved lysis of group N streptococci for isolation and rapid characterization of plasmid deoxyribonucleic acid.
Procedures for effective cellular lysis and plasmid deoxyribonucleic acid (DNA) isolation from group N streptococci were developed. Cells were grown at 32 degrees C for 4 h in a modified Elliker broth containing 20 mM DL-threonine. After cellular digestion with 2 mg of lysozyme per ml for 7 min at 3...
محفوظ في:
| الحاوية / القاعدة: | Appl Environ Microbiol |
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| المؤلفون الرئيسيون: | , , |
| التنسيق: | Artigo |
| اللغة: | Inglês |
| منشور في: |
American Society for Microbiology (ASM)
1978
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| الموضوعات: | |
| الوصول للمادة أونلاين: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC242884/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/416755/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.35.3.592-600.1978 |
| الوسوم: |
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