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Microscale method for rapid isolation of covalently closed circular plasmid DNA from group N streptococci.

A method for rapid purification of plasmid DNA from lactic streptococci, utilizing microliter quantities of reagents, was developed by combination of a short lysozyme-mutanolysin cell wall digestion with a modification of the Escherichia coli plasmid isolation procedure of McMaster et al. (Anal. Bio...

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Detalhes bibliográficos
Publicado no:Appl Environ Microbiol
Main Authors: Orberg, P K, Sandine, W E
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 1984
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC239747/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6426385/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.47.4.677-680.1984
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