Improved lysis of group N streptococci for isolation and rapid characterization of plasmid deoxyribonucleic acid.

Procedures for effective cellular lysis and plasmid deoxyribonucleic acid (DNA) isolation from group N streptococci were developed. Cells were grown at 32 degrees C for 4 h in a modified Elliker broth containing 20 mM DL-threonine. After cellular digestion with 2 mg of lysozyme per ml for 7 min at 3...

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Bibliographic Details
Published in:Appl Environ Microbiol
Main Authors: Klaenhammer, T R, McKay, L L, Baldwin, K A
Format: Artigo
Language:Inglês
Published: American Society for Microbiology (ASM) 1978
Subjects:
Research Article
Online Access:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC242884/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/416755/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.35.3.592-600.1978
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