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Poliovirus RNA-Dependent RNA Polymerase (3D(pol)): Pre-Steady-State Kinetic Analysis of Ribonucleotide Incorporation in the Presence of Mg(2+)
We have solved the complete kinetic mechanism for correct nucleotide incorporation catalyzed by the RNA-dependent RNA polymerase from poliovirus, 3D(pol). The phosphoryl-transfer step is flanked by two isomerization steps. The first conformational change may be related to reorientation of the tripho...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2004
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2426923/ https://ncbi.nlm.nih.gov/pubmed/15122878 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi035212y |
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