Gel-based oligonucleotide microarray approach to analyze protein–ssDNA binding specificity

Gel-based oligonucleotide microarray approach was developed for quantitative profiling of binding affinity of a protein to single-stranded DNA (ssDNA). To demonstrate additional capabilities of this method, we analyzed the binding specificity of ribonuclease (RNase) binase from Bacillus intermedius...

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Autors principals: Zasedateleva, Olga A., Mikheikin, Andrey L., Turygin, Alexander Y., Prokopenko, Dmitry V., Chudinov, Alexander V., Belobritskaya, Elena E., Chechetkin, Vladimir R., Zasedatelev, Alexander S.
Format: Artigo
Idioma:Inglês
Publicat: Oxford University Press 2008
Matèries:
Methods Online
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC2425478/
https://ncbi.nlm.nih.gov/pubmed/18474529
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkn246
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