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Chemical mapping of cytosines enzymatically flipped out of the DNA helix
Haloacetaldehydes can be employed for probing unpaired DNA structures involving cytosine and adenine residues. Using an enzyme that was structurally proven to flip its target cytosine out of the DNA helix, the HhaI DNA methyltransferase (M.HhaI), we demonstrate the suitability of the chloroacetaldeh...
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| Main Authors: | , , , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
Oxford University Press
2008
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2425465/ https://ncbi.nlm.nih.gov/pubmed/18450817 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkn200 |
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