Improved method for detecting poliovirus negative strands used to demonstrate specificity of positive-strand encapsidation and the ratio of positive to negative strands in infected cells.

We have developed a ribonuclease protection method suitable for sensitive detection of an RNA species in the presence of a large excess of its complementary strand, as for the detection of negative strands of positive-strand RNA viruses. By using this method to probe for poliovirus negative strands...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Veröffentlicht in:J Virol
Hauptverfasser: Novak, J E, Kirkegaard, K
Format: Artigo
Sprache:Inglês
Veröffentlicht: American Society for Microbiology (ASM) 1991
Schlagworte:
Research Article
Online Zugang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC241002/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1851886/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jvi.65.6.3384-3387.1991
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