Improved method for detecting poliovirus negative strands used to demonstrate specificity of positive-strand encapsidation and the ratio of positive to negative strands in infected cells.
We have developed a ribonuclease protection method suitable for sensitive detection of an RNA species in the presence of a large excess of its complementary strand, as for the detection of negative strands of positive-strand RNA viruses. By using this method to probe for poliovirus negative strands...
Sparad:
| I publikationen: | J Virol |
|---|---|
| Huvudupphov: | , |
| Materialtyp: | Artigo |
| Språk: | Inglês |
| Utgiven: |
American Society for Microbiology (ASM)
1991
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| Ämnen: | |
| Länkar: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC241002/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1851886/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jvi.65.6.3384-3387.1991 |
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